ELISA Bionesium(TM) Alkaline Phosphatase

Product Properties Optimal reaction temperature: 37℃. Definition of active unit: 1 active unit is defined as the amount of enzyme needed to dephosphorylate 1μg of pUC19 plasmid vector in 30m at 37℃ (dephosphorylation is defined as ＞95% inhibitory action on re-cyclization in the self-linking reaction, and measured throµgh transformation into E. coli.) Quality Control Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free. Product Features Dephosphorylation of DNA and RNA 5’ and 3’ termini. Can be used for DNA dephosphorylation before terminus-labelling by T4 polynucleotide kinase. Used for treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis. Reducing the immunogenicity of mRNA in mammals by dephosphorylating mRNA. Avoiding re-cyclization during the cloning process by dephosphorylating DNA cloning vector. No BSA system, less heat source contamination. -Quality System, Quality management Systems (QMS), ISO13485 or ISO9001-certified and GMP-compliant -All products have Certificate of Analysis (CoA) available. -Some products have Drµg Master Files (DMF FDA) Available -No animal origination attestation -Comparable with brand name products for activity, purity, impurity, residual host DNA/residual host protein etc. -ICH and pharmacopeia Please inquire with us: info@genebiosystems.com