PRECAST GEL

Precast gels are ready-to-use polyacrylamide or agarose gels designed for electrophoresis, eliminating the need for manual gel preparation in the laboratory. These gels come pre-cast in plastic cassettes with defined well configurations and concentrations, ensuring consistent performance and reproducibility across experiments.

They are primarily used for separating proteins (via SDS-PAGE or native PAGE) or nucleic acids (DNA/RNA) during electrophoresis. Precast gels improve workflow efficiency by reducing preparation time and minimizing variability due to manual casting. They are available in a range of acrylamide concentrations, gradient formats, and well counts to accommodate different sample types and resolutions.

Key benefits include:

• Time-saving setup for rapid electrophoresis
• Improved reproducibility and consistency
• Reduced risk of polymerization errors
• Long shelf life when stored properly
• Compatible with most standard gel tanks and blotting systems

Precast gels are widely used in routine protein analysis, Western blotting, nucleic acid separation, and high-throughput screening environments.

🧪 ATTO Precast Gel Lineup

 

1. e-PAGEL

• Size: Mini (90 mm x 83 mm)
• Application: Ideal for protein and DNA electrophoresis, including SDS-PAGE and native PAGE
• Features:
  • Stable and reproducible separation
  • Compatible with ATTO Mini PAGE systems
  • Available in various concentrations (e.g., 7.5%, 10%, 12.5%, 15%, 5–20%)
  • Options for 14-well, 18-well, and well-less formats for 2D electrophoresis
  • Does not contain SDS; suitable for both SDS-PAGE and native PAGE by selecting appropriate running buffers

 

2. c-PAGEL

• Size: Compact (60 mm x 60 mm)
• Application: Designed for rapid electrophoresis of low molecular weight proteins and polypeptides
• Features:
  • Fast run times, as short as 10 minutes
  • High-speed migration capabilities
  • Suitable for quick analyses and time-sensitive experiments

 

3. agarGEL

• Application: Used for isoelectric focusing (IEF) in the first dimension of 2D gel electrophoresis
• Features:
  • No need for swelling treatment; ready for immediate use
  • Capable of separating proteins with high molecular weights (100–250 kDa)
  • Gels can be stored at -20°C for up to one month, allowing for subsequent second-dimension electrophoresis
  • Compatible with ATTO 2D Electrophoresis Systems

4. U-PAGEL

• Application: Optimized for the separation of high molecular weight proteins
• Features:
  • Enhanced elasticity to accommodate larger proteins
  • Provides clear resolution for proteins that are challenging to separate using standard gels

5. m-PAGEL

• Application: Suitable for high-throughput analyses requiring multiple samples
• Features:
  • Wide gel format with up to 30 wells per gel
  • Facilitates simultaneous processing of numerous samples