ClonExpress Ultra One Step Cloning Kit
High-Efficiency Homologous Recombination for Seamless DNA Assembly.
The ClonExpress Ultra One Step Cloning Kit is a next-generation recombinant DNA cloning solution that supports seamless and ligase-independent homologous recombination for 1 to 5 DNA fragments. Powered by a highly optimized 2× ClonExpress Mix—which includes the enhanced Exnase recombinase—this kit offers superior recombination efficiency, minimal background, and enhanced impurity tolerance.
The included pCE-Zero vector is compatible with most PCR products, enabling direct use of PCR amplicons without restriction digestion or pretreatment, streamlining the cloning workflow.
🔬 Key Features
- All-in-One Simplicity: Single-tube, ready-to-use super mix
- Rapid Workflow: Complete recombination in 5–15 minutes
- High Efficiency: Clone fragments from 50 bp to 10 kb with a positive rate >95%
- Flexible Compatibility: Supports single-fragment, multi-fragment, and Entry cloning
- Low Background: Dual-selection pCE-Zero vector (Amp/Kan resistant) with CcdB negative selection
- Smart Design: Access to CE Design—an intuitive, online primer design tool
🧪 Kit Components
- 2× ClonExpress Mix (with Exnase-enhanced recombinase)
- pCE-Zero vector with dual selection and CcdB gene
- User manual and online resources