Se rendre au contenu

CloneExpress Ultra One Step Cloning Kit V3

ClonExpress Ultra One Step Cloning Kit V3 offers a streamlined and powerful solution for seamless DNA assembly. Engineered with the latest optimized homologous recombination technology, this advanced kit enables rapid and efficient assembly of 1 to 11 DNA fragments with overlaps ranging from 15 to 35 bp, making it ideal for complex cloning applications involving large vectors or GC-rich/poor homologous arms.

CloneExpress Ultra One Step Cloning Kit V3

Key Features

  • High Cloning Efficiency
    Achieves a >95% positive rate, even in multi-fragment assembly (up to 11 fragments).
  • Fast Reaction Time
    Complete cloning in just 5–30 minutes, allowing for quicker downstream workflows.
  • Broad Compatibility
    Performs reliably with low-input DNA, GC-rich or GC-poor overlaps, and low-purity templates.
  • Optimized 2× CE Mix V3
    Ensures robust cloning success by improving compatibility with varying DNA concentrations and sequence complexity.
  • Flexible Overlap Requirements
    Supports overlaps from 15 to 35 bp, exceeding the typical 15–20 bp range.

Kit Components

  • 2× CE Mix V3
  • Positive Control DNA
  • Detailed protocol booklet

Storage Conditions

  • Store at -30°C to -15°C
  • Transport at ≤0°C