ELISA CHEK2 (Ab-68) anti-

Quantity :100µL Clone Number: Aliases:CDS 1 antibody; Cds1 antibody; Cds1 homolog antibody; Checkpoint kinase 2 antibody; Checkpoint like protein CHK2 antibody; CHEK 2 antibody; Chek2 antibody; Chk 2 antibody; CHK2 checkpoint homolog (S. pombe) antibody; CHK2 checkpoint homolog antibody; CHK2_ antibody; hCds1 antibody; HuCds 1 antibody; LFS 2 antibody; LFS2 antibody; PP1425 antibody; RAD 53 antibody; RAD53 antibody; Rad53 homolog antibody; Serine/threonine protein kinase Chk2 antibody; Serine/threonine-protein kinase Chk2 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:O96017 Immunogen:SyntheQuantityd non-phosphopeptide derived from Chk2 around the phosphorylation site of threonine 68. Raised in:Rabbit Reactivity:, Mouse, Rat Tested Applications:ELISA, WB, IHC; WB:1:500-1:3000, IHC:1:50-1:100 Background:Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regµLate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. RegµLates cell cycle checkpoint arrest throµgh phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. RegµLates DNA repair throµgh phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimµLates the transcription of genes involved in DNA repair (including BRCA2) throµgh the phosphorylation and activation of the transcription factor FOXM1. RegµLates apoptosis throµgh the phosphorylation of p53/TP53, MDM4 and PmL. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumµLation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes throµgh phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Bhoumik A, et al. (2005) Mol Cell; 18(5): 577-87. GorgoµLis VG, et al. (2005) Nature; 434(7035): 907-13. Falck J, et al. (2005) Nature; 434(7033): 605-11. Jin ZH, et al. (2005) Oncogene; 24(12): 1973-81. Li J, et al. (2005) J Biol Chem; 280(12): 12041-50. Clonality:Polyclonal Isotype:IgG Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:CHEK2 Research Areas:Epigenetics and Nuclear Signaling;Cancer