ELISA b-Enolase anti-

Quantity:100µL Host:Rabbit Clonality:Polyclonal Isotype:IgG GeneName:ENO3 GeneID:2027 Uniprot:P13929 Specificity:b-Enolase Antibody detects endogenous levels of total b-Enolase. Immunogen:A syntheQuantityd peptide derived from b-Enolase(ENO-3), corresponding to a region within C-terminal amino acids. Storage:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Reactivity:,Mouse Prediction:Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus Application:WB,IHC,IF/ICC,ELISA(peptide) Dilution:WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000 Conjµgate:Unconjµgated RRID:AB_2835357 An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide:AF4099-BP Des# Blocking Peptide:b-Enolase Blocking peptide Blocking Peptide Price:$549 CAD