ELISA Lipoarabinomannan (LAM)

Reactivity: (Homo sapiens) UniProt:N/A Abbreviation:LAM Alternative Names:N/A Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.9% Inter-AssayCV:?8.7% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LAM in samples. An antibody specific for LAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LAM is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAM bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Lipoarabinomannan is a lipoglycan and major virµLence factor in the bacteria genus Mycobacterium. In addition to serving as a major cell wall component, it is thoµght to serve as a modµLin with immunoregµLatory and anti-inflammatory effects. This allows the bacterium maintain survival in the reservoir by undermining host resistance and acquired immune responses. These mechanisms include the inhibition of T-cell proliferation and of macrophage microbicidal activity via diminished IFN-? response. Additional functions of lipoarabinommanan are thoµght to include the neutralization of cytotoxic oxygen free radicals produced by macrophages, inhibition of protein kinase C, and induction of early response genes. Lipoarabinomannan is syntheQuantityd via addition of mannose residues to phosphoinositol by a series of mannosyltransferases to produce PIMs and lipomannan(LM). Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).