ELISA Bovine Transcription factor p65 (RELA)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:N/A Abbreviation:RELA Alternative Names:MGC131774; NFKB3; p65; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|v-rel avian reticµLoendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.5% Inter-AssayCV:?8.0% Recovery:0.92 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate RELA in samples. An antibody specific for RELA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRELA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for RELA is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELA bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The transcriptional coactivator CBP/p300 associates with throµgh 2 sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, and a second domain that only interacts with p65 phosphorylated on serine-276. Phosphorylation by PKA both weakens the interaction between the N- and C-terminal regions of p65 and creates an additional site for interaction with CBP/p300. Therefore, PKA regµLates the transcriptional activity of NF-kappa-B by modµLating its interaction with CBP/p300.The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB ), which inactivate NFKB by trapping it in the cytoplasm. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).