ELISA Protein maelstrom homolog (MAEL)

Reactivity:Pig (Sus scrofa; Porcine) UniProt:A4UTQ0 Abbreviation:MAEL Alternative Names:FLJ14904; RP11-102C16.1; maelstrom homolog Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?7.8% Inter-AssayCV:?7.9% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate MAEL in samples. An antibody specific for MAEL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAEL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for MAEL is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAEL bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:By excision of a P element insertion in the 5-prime UTR of the Drosophila maelstrom gene, Findley et al. (2003) isolated mael(M391), a nµLl allele of Drosophila maelstrom. They used database analysis to identify the MAEL homolog. Confocal microscopy localized Drosophila maelstrom primarily to nuage, highly abundant particles within germLine cells, and also to the nucleus and cytoplasm of germLine cells. Nuclear shuttling assays showed that Drosophila maelstrom is transported between the cytoplasm and nucleus.By phenotypic characterization of Drosophila maelstrom mutants, which displayed axial patterning defects and other polarity defects, Findley et al. (2003) defined maelstrom as a spindle-class gene that affects Vasa modification. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).