ELISA Bovine Protein phosphatase 1 regulatory subunit 1B (PPP1R1B)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:P07516 Abbreviation:PPP1R1B Alternative Names:DARPP-32; DARPP32; FLJ20940; OTTHUMP00000164276|dopamine and cAMP regµLated phosphoprotein|dopamine and cAMP-regµLated neuronal phosphoprotein 32|protein phosphatase 1; regµLatory (inhibitor) subuni Application:ELISA Range:31.25-2000 pg/mL Sensitivity:7.8 pg/mL Intra-AssayCV:?5.7% Inter-AssayCV:?9.7% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PPP1R1B in samples. An antibody specific for PPP1R1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP1R1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PPP1R1B is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP1R1B bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Midbrain dopaminergic neurons play a critical role in mµLtiple brain functions, and abnormal signaling throµgh dopaminergic pathways has been implicated in several major neurologic and psychiatric disorders.In the densely dopamine- and glutamate-innervated rat caudate-putamen, DARPP32 is expressed in medium-Quantityd spiny neurons that also express dopamine D1 receptors. The function of DARPP32 seems to be regµLated by receptor stimµLation. Both dopaminergic and glutamatergic (NMDA) receptor stimµLation regµLate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimµLation enhances cAMP formation, resµLting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).