ELISA Bovine Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:P35705 Abbreviation:PRDX3 Alternative Names:AOP-1; AOP1; MER5; MGC104387; MGC24293; PRO1748; SP-22; antioxidant protein 1|thioredoxin-dependent peroxide reductase Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.8% Inter-AssayCV:?7.8% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PRDX3 in samples. An antibody specific for PRDX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PRDX3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:PRDX3 encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The and mouse genes are highly conserved, and they map to the regions syntenic between mouse and chromosomes. Sequence comparisons with recently cloned mammalian homologues sµggest that these genes consist of a family that is responsible for regµLation of cellµLar proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).