ELISA Prolyl endopeptidase (PREP)

Reactivity:Pig (Sus scrofa; Porcine) UniProt:P23687 Abbreviation:PREP Alternative Names:RP3-355L5.1; MGC16060; PE; PEP; dJ355L5.1 (prolyl endopeptidase)|post-proline cleaving enzyme|prolyl oligopeptidase Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.3% Inter-AssayCV:?9.7% Recovery:0.86 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PREP in samples. An antibody specific for PREP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPREP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PREP is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PREP bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Prolyl endopeptidase (PE) also known as prolyl oligopeptidase or post-proline cleaving enzyme is an enzyme that in s is encoded by the PREP gene.Prolyl endopeptidase is a large cytosolic enzyme that belongs to a distinct class of serine peptidases. It was first described in the cytosol of rabbit brain as an oligopeptidase, which degrades the nonapeptide bradykinin at the Pro-Phe bond.[5] The enzyme is involved in the maturation and degradation of peptide hormones and neuropeptides such as alpha-melanocyte-stimµLating hormone, luteinizing hormone-releasing hormone (LH-RH), thyrotropin-releasing hormone, angiotensin, neurotensin, oxytocin, substance P and vasopressin. PREP cleaves peptide bonds at the C-terminal side of proline residues. Its activity is confined to action on oligopeptides of less than 10 kD and it has an absolute requirement for the trans-configuration of the peptide bond preceding proline. Prolyl endopeptidases are involved in the maturation and degradation of peptide hormones and neuropeptides. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).