ELISA Bovine Prostaglandin reductase 2 (PTGR2)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:Q32L99 Abbreviation:PTGR2 Alternative Names:DKFZp686P10120; FLJ39091; FLJ99229; PGR2; ZADH1; 15-oxoprostaglandin-delta13-reductase|zinc binding alcohol dehydrogenase; domain containing 1 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?6.9% Inter-AssayCV:?8.9% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PTGR2 in samples. An antibody specific for PTGR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PTGR2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGR2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:PTGR2 is an enzyme involved in the metabolism of prostaglandins. The encoded protein catalyzes the NADPH-dependent conversion of 15-keto-prostaglandin E2 to 15-keto-13,14-dihydro-prostaglandin E2. This protein may also be involved in regµLating activation of the peroxisome proliferator-activated receptor. Alternative splicing resµLts in mµLtiple transcript variants. ZADH1 has a domain characteristic of the zinc-containing ADH family , a proline-rich motif, and a putative NADP(+)-binding domain. RT-PCR detected ubiquitous expression of the shorter ZADH1 transcript (ZADH1b), with highest expression in kidney, liver, pancreas, prostate, and heart, followed by lung, skeletal muscle, testis, small intestine, and brain. Expression was low in all other tissues examined. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).