ELISA DCLRE1C anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobµLins and T-cell receptor proteins are assembled from individual V,(D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks(DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining(NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ. Immunogen:DNA cross-link repair 1C(PSO2 homolog, S. cerevisiae) Synonyms:ARTEMIS, ASCID, SCIDA, SNM1C Observed MW:60 kDa Uniprot ID:Q96SD1 Reactivity:, Mouse, Rat Tested Application:ELISA, WB Recommended dilution:WB: 1:500-1:2000 Gene ID:64421 Research Area:Metabolism