HiScript IV RT SuperMix for qPCR (+gDNA wiper)
High-efficiency qPCR cDNA synthesis kit with gDNA removal. Ideal for low-input or degraded RNA. One-step reverse transcription in minutes.
HiScript IV RT SuperMix for qPCR (+gDNA wiper) is an enhanced, next-generation reverse transcription system engineered for high-performance quantitative PCR (qPCR). Based on the proven HiScript III technology, this upgraded kit features the innovative HiScript IV Reverse Transcriptase and an optimized buffer system to significantly boost cDNA synthesis efficiency, even from challenging RNA samples.
This one-tube system includes a powerful 5× gDNA wiper Mix for complete removal of genomic DNA contamination, eliminating the need for intron-spanning primers and improving the precision of gene expression analysis. The 4× HiScript IV qRT SuperMix is pre-formulated with all necessary reagents, enabling a streamlined workflow—simply add RNA and RNase-free water.
Key Features
✅ Superior Reverse Transcription Efficiency
Delivers high yields of full-length cDNA and lower Ct values for enhanced qPCR sensitivity and reproducibility.
✅ Efficient gDNA Removal
The 5× gDNA wiper Mix thoroughly eliminates up to 500 ng of genomic DNA, ensuring data integrity and eliminating false amplification signals.
✅ Broad Template Compatibility
Optimized for low-input RNA, low-abundance transcripts, and degraded RNA, including samples from FFPE tissues or clinical isolates.
✅ Convenient One-Step Protocol
All-in-one formulation reduces handling time and pipetting steps—ideal for high-throughput or time-sensitive experiments.
Ideal For :
- Gene expression analysis
- Low-expression RNA studies
- Clinical diagnostics and biomarker validation
- RNA from difficult samples (e.g., FFPE, serum/plasma, degraded tissues)
Storage & Stability :
- Store at –30°C to –15°C
- Transport at ≤0°C
- Stable for long-term use under recommended conditions
Superior Reverse Transcription Efficiency
100 ng of RNA from animal, plant, and microbial sources was reverse transcribed using Gentaur GENR-423-01 and comparable RT kits from Supplier A, Supplier B, and Supplier C, following each manufacturer’s protocol. Reverse transcription efficiency was assessed via qPCR amplification of the resulting cDNA.
As shown in the figure, the ΔCt between Gentaur GENR-423-01 and Supplier C is within 0.5, indicating similar RT efficiency. In contrast, the ΔCt values between Gentaur GENR-423-01 and Supplier A or Supplier B exceed 0.5, demonstrating that Gentaur GENR-423-01 provides higher reverse transcription efficiency in comparison.
