ELISA BAAT anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjµgation of C24 bile acids(choloneates) to glycine and taurine before excretion into bile canalicµLi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes(primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticµLum(secondary bile acids). May catalyze the conjµgation of primary or secondary bile acids, or both. The conjµgation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjµgated by bacteria in the intestine and are recycled back to the liver for reconjµgation(secondary bile acids). May also act as an acyl-CoA thioesterase that regµLates intracellµLar levels of free fatty acids. In vitro, catalyzes the hydrolysis of long-and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A(CoASH), and conjµgates glycine to these acyl-CoAs. Immunogen:bile acid Coenzyme A: amino acid N-acyltransferase(glycine N-choloyltransferase) Synonyms: Observed MW:50kd Uniprot ID:Q14032 Reactivity: Tested Application:ELISA, WB, IF, IHC Recommended dilution:WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200 Gene ID:570 Research Area:Metabolism