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B-27™ Plus Supplement (50X)

Advanced Serum-Free Supplement for Neuronal Cell Culture & Stem Cell Research

The B-27 Plus Supplement (50X) is a next-generation, serum-free cell culture supplement specifically formulated to support the growth, survival, and functional maturation of neuronal cells. Designed for high-performance research applications, this optimized supplement ensures enhanced viability and reproducibility in both primary neurons and stem cell-derived neuronal cultures.

Key Features & Benefits

  • Serum-free formulation : Eliminates variability associated with serum-based media.
  • Enhanced neuronal survival : Up to 50% higher survival rates vs. traditional formulations.
  • Optimized composition : Improved electrophysiological activity and neuronal maturation.
  • Promotes neurite outgrowth : Supports synapse formation and network development.
  • High reproducibility : Strict quality control for consistent experimental outcomes.
  • Ready-to-use 50X concentrate : Easy dilution and integration into workflows.

Technical Specifications

  • Product Name: B-27 Plus Supplement
  • Catalog Number: Check here
  • Concentration: 50X
  • Volume: 10 mL (typical format).
  • Form: Liquid, sterile-filtered.
  • Type: Serum-free supplement.
  • Cell Type: Neural cells (primary neurons, stem cell-derived neurons).
  • Storage: -5°C to -20°C, protect from light.
  • Shelf Life: ~12 months.

Product Description

B-27 Plus Supplement is an optimized upgrade of the widely cited B-27 formulation, engineered to provide superior neuronal cell culture performance. It supports:

  • Long-term survival of primary rodent neurons.
  • Growth of human stem cell-derived neurons.
  • Improved synaptic activity and network stability.

Applications

Neuroscience Research

  • Primary neuronal cell culture.
  • CNS neuron maintenance.
  • Synaptic activity studies.

Stem Cell & Regenerative Medicine

  • Differentiation of pluripotent stem cells into neurons.
  • Organoid and 3D neuronal models.

Dr ug Discovery & Toxicology

  • Neurotoxicity testing.
  • Screening of CNS-active compounds.

Biotechnology & Academic Research

  • Electrophysiology studies.
  • Neural network modeling.
  • Disease modeling (Alzheimer’s, Parkinson’s, etc.).

Why Choose B-27 Plus Supplement?

B-27 Plus Supplement stands out as a gold-standard neuronal culture additive, offering:

  • Higher consistency than traditional serum-containing systems.
  • Improved maturation and functionality of neurons.
  • Compatibility with a wide range of neural cell types.

Its optimized formulation helps reduce experimental variability while increasing the reliability of results in advanced research environments.

Composition Overview

B-27 supplements typically contain a complex mix of nutrients and protective factors, including :

  • Antioxidants to reduce oxidative stress.
  • Vitamins and hormones.
  • Lipids and essential growth factors.

These components work together to support neuronal viability, reduce cellular stress, and enhance differentiation and growth.

Storage & Handling

  • Store at -20°C to -5°C.
  • Avoid repeated freeze-thaw cycles.
  • Thaw at 4°C before use.
  • Protect from light to maintain stability.