BsaI, GMP Grade - 100 KU
High-Purity Restriction Enzyme for Advanced Molecular Biology & GMP Workflows
The BsaI restriction enzyme (GMP grade) is a high-performance Type IIS endonuclease designed for precision DNA manipulation in research, biotechnology, and regulated production environments. Manufactured under stringent GMP conditions, this enzyme ensures reproducibility, safety, and compliance for critical applications including gene editing, plasmid construction, and synthetic biology workflows.
Derived from Bacillus thermophilus, BsaI recognizes asymmetric DNA sequences and cleaves outside its recognition site, enabling seamless DNA assembly and advanced cloning strategies.
Key Features & Benefits
- GMP-grade quality : Manufactured under strict Good Manufacturing Practice standards.
- High enzymatic activity : Reliable digestion performance for consistent results.
- Type IIS restriction enzyme : Cuts outside recognition site for flexible DNA design.
- Optimized for precision cloning : Ideal for Golden Gate Assembly and modular cloning.
- Low contamination profile : Minimal nuclease and endotoxin interference.
- Reproducible performance : Suitable for research and industrial-scale workflows.
Technical Specifications
- Product Name : BsaI, GMP Grade
- Catalog Number : GMP-RE026
- Size : 100 KU
- Recognition Sequence :
- 5’…GGTCTC(N)1↓…3’
- 3’…CCAGAG(N)5↑…
- Cleavage Type : Type IIS (cuts outside recognition sequence)
- Source Organism : Bacillus thermophilus
- Format : Liquid enzyme preparation
Applications
Molecular Biology & Genetic Engineering
- Golden Gate Assembly
- DNA cloning and subcloning
- Plasmid linearization
- Gene synthesis workflows
Biotechnology & Industrial Use
- mRNA and DNA production pipelines
- Synthetic biology platforms
- CRISPR and gene editing preparation
Pharmaceutical & GMP Environments
- Vaccine development workflows
- Biopharmaceutical manufacturing
- Regulated enzyme-based processing
Why Choose GMP-Grade BsaI ?
Using GMP-grade enzymes is essential for workflows requiring traceability, regulatory compliance, and batch-to-batch consistency. This BsaI enzyme is specifically designed to meet the demands of:
- Clinical research and translational studies.
- Biotech and pharmaceutical production.
- High-throughput molecular biology labs.
Its precise cleavage pattern enables scarless DNA assembly, reducing errors and improving efficiency in complex constructs.
Storage & Handling
- Storage Temperature : -20°C
- Handling : Avoid repeated freeze-thaw cycles.
- Shipping : Delivered under controlled conditions to maintain activity.