QuantStudio™ Absolute Q™ Digital PCR System
QuantStudio™ Absolute Q™ Digital PCR System
The Next‑Generation dPCR Platform
The QuantStudio™ Absolute Q™ Digital PCR System is a powerful digital PCR (dPCR) instrument designed to deliver absolute nucleic acid quantification with industry‑leading sensitivity, precision, and workflow simplicity. It blends cutting‑edge microfluidic technology with a streamlined, qPCR‑like operation to empower researchers with faster, more accurate data in challenging genomic applications.
What Is Digital PCR (dPCR)?
Digital PCR is a next‑generation molecular technique that partitions a DNA or RNA sample into thousands of independent microreactions. Each partition behaves as an individual PCR reaction, enabling absolute quantification of target nucleic acids without the need for standard curves. This method offers enhanced precision, sensitivity, and reproducibility compared to traditional qPCR, especially in low‑abundance or complex samples.
Key Features and Benefits
1. Simple, Integrated Digital PCR Workflow
The Absolute Q system simplifies digital PCR into a single integrated workflow from sample digitization to thermal cycling and data acquisition all within one instrument. The intuitive process typically requires under five minutes of hands‑on time, significantly reducing setup complexity and human error.
2. Microfluidic Array Plate (MAP) Technology
At the core of the Absolute Q system is patented microfluidic array plate (MAP) technology, which partitions samples into multiple thousands of micro‑chambers for digital amplification. This design enables :
- Over 95% sample utilization per reaction (compared to 25–60% in many alternatives), leading to more reliable and sensitive measurements.
- Exceptional consistency and reproducibility of results.
3. Fast Time to Results
The system integrates all critical steps into one device. Users can complete a full digital PCR run including reagent digitization, thermal cycling, and data analysis often in under 90 minutes, helping boost lab productivity.
4. High Sensitivity and Precision
The Absolute Q system detects even rare genetic targets and low‑copy nucleic acids with high confidence. It supports up to four optical channels for multiplexing, enabling simultaneous analysis of multiple genomic targets in a single run.
5. Versatile Research Applications
This state‑of‑the‑art dPCR platform supports a wide range of applications in molecular biology and biomedical research:
- Rare mutation detection and liquid biopsy studies.
- Copy number variant analysis.
- Absolute gene expression quantification.
- Genotyping and pathogen detection.
- Cell and gene therapy development workflows.
These applications benefit from the precision of absolute quantification inherent in digital PCR technology..
How QuantStudio Absolute Q Works?
- Prepare Reaction Mix : Combine sample DNA/RNA with PCR reagents and load into a MAP16 digital PCR plate.
- Partition Sample : Proprietary microfluidic technology divides the reaction into thousands of micro‑chambers.
- Thermal Cycling : PCR amplification occurs within each chamber.
- Absolute Quantification : The system counts positive and negative reactions to calculate target concentration directly, without standard curves a key benefit of digital PCR.
Software and Data Analysis
The QuantStudio Absolute Q system includes intuitive software that helps streamline experiment setup, monitor runs, and analyze data with quality control metrics, confidence interval calculations, and advanced reporting tools suitable for both small and large‑scale studies.
Why Researchers Choose Absolute Q?
- Precision: Absolute quantification of targets without calibration curves.
- Speed: Fast workflow and rapid data generation in under 90 minutes.
- Ease of Use: Minimal hands‑on time, qPCR‑like workflow.
- Sample Efficiency: Analyzes >95% of sample input thanks to microfluidic design.
Additional Tools and Upgrades
For labs requiring higher throughput, the Absolute Q AutoRun Digital PCR Suite extends productivity with automated plate handling and walk‑away operation, enabling multi‑day unattended digital PCR runs and increased sample capacity.
The QuantStudio Absolute Q Digital PCR System represents a leading platform in digital PCR technology, delivering absolute quantification with unmatched ease, speed, and consistency. Whether in oncology research, genomics, environmental testing, or therapeutic development, this system empowers scientists to quantify nucleic acids with confidence and precision.