ELISA Bionesium(TM) Proteinase K

Technical Data Appearance White amorphous lyophilized powder Protein purity ≥95% Specific activity ≥30 U/mg Protein Deoxyribonuclease activity No activity of deoxyribonuclease was detected Ribonuclease activity No activity of Ribonuclease was detected Enzymatic Properties Source Tritirachium album Classification EC 3.4.21.64 MolecµLar weight 29 kDa (SDS-PAGE) Isoelectric point 7.81 Optimal pH 7.0-12.0 Both have high activity Char1 Optimum temperature 65 ℃ Chart2 pH stability pH 4.5-12.5 (25 ℃, 16 h) Chart3 Thermal stability Stable below 50 ℃（pH 8.0, 30 min） Chart4 Activator SDS, Urea Inhibitor Diisopropylfluorophosphate (DFIP)，BenzenesµLfonyl fluoride (PMSF) Storage conditions Dry powder can be stored at - 20 ℃ for a long time; After dissolving, it shoµLd be packed into proper volume, stored at 2-8 ℃ for a short time, and stored at - 20 ℃ for a long time. Use: The virus consists of a protein shell and an internal nucleic acid. To amplify the nucleic acid by PCR, the shell protein must be destroyed first. The role of protease K is to break down the protein shell of the virus, releasing nucleic acid for easy detection. 1. Gene diagnosis kit；2. RNA and DNA Extraction Kit；3. Extract non protein components from tissues and degrade protein impurities. For example, the preparation of DNA vaccine；4. Preparation of chromosome DNA by pµLse electrophoresis；5. Western blotting；6. R&D and mass production of enzyme based glycated albumin reagents for in vitro diagnosis.