ELISA CHFR anti-

Quantity :100µL Clone Number: Aliases:Checkpoint with forkhead and ring finger domains antibody; Checkpoint with forkhead and RING finger domains protein antibody; Chfr antibody; CHFR_ antibody; E3 ubiquitin protein ligase CHFR antibody; E3 ubiquitin-protein ligase CHFR antibody; EC 6.3.2. antibody; FLJ10796 antibody; FLJ33629 antibody; RING finger protein 196 antibody; RNF 116 antibody; RNF 196 antibody; RNF116 antibody; RNF196 antibody; Ubiquitin ligase protein antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q96EP1 Immunogen:SyntheQuantityd peptide derived from Internal of CHFR. Raised in:Rabbit Reactivity:, Mouse, Rat Tested Applications:ELISA, WB; WB:1:500-1:3000 Background:E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubµLe poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubµLe poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjµgating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellµLar stress. Scolnick D.M., Nature 406:430-435(2000). Ota T., Nat. Genet. 36:40-45(2004). Scherer S.E., Nature 440:346-351(2006) Clonality:Polyclonal Isotype:IgG Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:CHFR Research Areas:Epigenetics and Nuclear Signaling;Cancer;Cell biology