ELISA ALYREF anti-

Quantity :100µL Clone Number: Aliases:Ally of AmL-1 and LEF-1 antibody; Ally of AmL1 and LEF1 antibody; ALY antibody; ALY/REF antibody; Aly/REF export factor antibody; BEF antibody; bZIP enhancing factor antibody; bZIP-enhancing factor BEF antibody; REF antibody; THO complex 4 antibody; THO complex subunit 4 antibody; Tho4 antibody; thoc4 antibody; THOC4_ antibody; Transcriptional coactivator Aly/REF antibody; Transcriptional coactivator antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q86V81 Immunogen:SyntheQuantityd peptide derived from internal of THOC4. Raised in:Rabbit Reactivity: Tested Applications:ELISA, WB; WB:1:500-1:3000 Background:Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thoµght to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thoµght to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability. Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation. Zody M.C., Nature 440:1045-1049(2006). The MGC Project Team, Genome Res. 14:2121-2127(2004). Clonality:Polyclonal Isotype:IgG Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:ALYREF Research Areas:Epigenetics and Nuclear Signaling