ELISA CHEK1 (Ab-317) anti-

Quantity :100µL Clone Number: Aliases:C85740 antibody; Cell cycle checkpoint kinase antibody; Checkpoint ; S. pombe; homolog of; 1 antibody; Checkpoint kinase 1 antibody; Checkpoint kinase 1 homolog (S. pombe) antibody; CHEK 1 antibody; Chek1 antibody; Chk 1 antibody; Chk1 antibody; CHK1 checkpoint homolog (S. pombe) antibody; CHK1_ antibody; EC 2.7.11.1 antibody; rad27 antibody; Serine/threonine protein kinase Chk1 antibody; Serine/threonine-protein kinase CHK1 antibody; STT3; subunit of the oligosaccharyltransferase complex; homolog A (S. cerevisiae) antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:O14757 Immunogen:Peptide sequence around aa.315~319 (S-S-S-Q-P) derived from Chk1. Raised in:Rabbit Reactivity:, Rat Tested Applications:ELISA, WB, IHC; WB:1:500-1:1000, IHC:1:50-1:200 Background: Chek1 is a protein kinase that inhibits mitotic entry after DNA damage, required for the DNA damage checkpoint and is strongly similar to murine Chek1. Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The mouse and proteins share 90% sequence identity throµgh the protein kinase domains. The sequence of the 476-amino acid Chek1 protein is 29%, 40%, and 44% identical to those of the fission yeast Chek1, C. elegans Chek1, and Drosophila 'grapes' (Grp) proteins, respectively. Chek1 is expressed ubiquitously as an approximately 2.4-kb mRNA, with the most abundant expression in thymus, testis, small intestine, and colon. The protein has altered mobility when isolated from cells treated with ionizing radiation, indicating that Chek1 is modified in response to DNA damage. In vitro, Chek1 directly phosphorylates a regµLator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, Chek1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-Cyclin-B complex and mitotic entry Zhang YW, et al. (2005) Mol Cell; 19(5): 607-18. Bhoumik A, et al. (2005) Mol Cell; 18(5): 577-87. Rocha S, et al. (2005) EMBO J. Clarke CA, et al. (2005) Biochem J. Yu X, et al. (2004) Mol Cell Biol; 24(21): 9478-86. Clonality:Polyclonal Isotype:IgG Purification Method:Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjµgates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. Conjµgate:Non-conjµgated Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:CHEK1 Research Areas:Epigenetics and Nuclear Signaling;Cancer