ELISA Rabbit Complement 3 (C3)

Reactivity:Rabbit (Oryctolagus cunicµLus) UniProt:P12247 Abbreviation:C3 Alternative Names:AHUS5; ARMD9; ASP; CPAMD1; acylation-stimµLating protein cleavage product|complement component C3 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.3% Inter-AssayCV:?9.4% Recovery:0.93 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate C3 in samples. An antibody specific for C3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for C3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Complement C3 is the major plasma proteins of the immune system complement pathways. The synthesis of these proteins is increased in response to inflammation and infection but at a slower rate than for traditional acute phase proteins. Complement protein C3 is the fourth component to attach in the complement reaction sequence. It is a beta-globµLin with a sedimentation coefficient of 5.5 and a molecµLar weight of 185,000. C3 is a central molecµLe in the complement system whose activation is essential for all the important functions performed by this system. C3 can promote phagocytosis during an inflammatory response against pathogens, but unregµLated activation of C3 coµLd lead to host cell damage. Low level of serum C3 associates with hypocomplementaemia, primary biliary cirrhosis Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).