ELISA Bovine CAMP-dependent protein kinase catalytic subunit beta (PRKACB)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:P05131 Abbreviation:PRKACB Alternative Names:RP11-82H13.1; DKFZp781I2452; MGC41879; MGC9320; PKACB; PKA C-beta|cAMP-dependent protein kinase catalytic beta subunit isoform 4ab|cAMP-dependent protein kinase catalytic subunit beta|protein kinase Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.6% Inter-AssayCV:?9.3% Recovery:0.92 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PRKACB in samples. An antibody specific for PRKACB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKACB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PRKACB is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKACB bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:cAMP is a signaling molecµLe important for a variety of cellµLar functions. cAMP exerts its effects by activating the AMP-activated protein kinase (AMPK), which transduces the signal throµgh phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regµLatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regµLatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regµLatory subunits and three catalytic subunits of AMPK have been identified in s. PRKACB is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. Three alternatively spliced transcript variants encoding distinct isoforms have been observed. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).