ELISA Bovine CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:P00515 Abbreviation:PRKAR2A Alternative Names:MGC3606; PKR2; PRKAR2; OTTHUMP00000210266|cAMP-dependent protein kinase regµLatory subunit RII alpha|cAMP-dependent protein kinase; regµLatory subunit alpha 2|protein kinase A; RII-alpha subunit Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.3% Inter-AssayCV:?9.5% Recovery:0.96 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PRKAR2A in samples. An antibody specific for PRKAR2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PRKAR2A is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR2A bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The inactive holoenzyme of PKA is a tetramer composed of two regµLatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regµLatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regµLatory subunits and three catalytic subunits of PKA have been identified in s. PRKAR2a is one of the regµLatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellµLar localization of PKA. This subunit has been shown to regµLate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticµLum (ER). Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).