ELISA Bovine Protein tyrosine phosphatase type IVA 3 (PTP4A3)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:A2VDT1 Abbreviation:PTP4A3 Alternative Names:PRL-3; PRL-R; PRL3; potentially prenylated protein tyrosine phosphatase Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.3% Inter-AssayCV:?9.7% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PTP4A3 in samples. An antibody specific for PTP4A3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTP4A3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PTP4A3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTP4A3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Protein tyrosine phosphatase type IVA 3 is an enzyme belongs to a small class of prenylated protein tyrosine phosphatases (PTPs). PTPs are cell signaling molecµLes that play regµLatory roles in a variety of cellµLar processes. This class of PTPs contain a PTP domain and a characteristic C-terminal prenylation motif. Studies of this class of PTPs in mice demonstrated that they were prenylated proteins in vivo, which sµggested their association with cell plasma membrane. Overexpression of this gene in mammalian cells was reported to inhibit angiotensin-II induced cell calcium mobilization and promote cell growth. Two alternatively spliced variants exist.Mainly expressed in cardiomyocytes and skeletal muscle; also found in pancreas. Consistently overexpressed in colon cancer metastasis. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).