ELISA Bovine Secretory immunoglobulin A (sIgA)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:N/A Abbreviation:sIgA Alternative Names:N/A Application:ELISA Range:0.781-50 ng/mL Sensitivity:0.34 ng/mL Intra-AssayCV:?5.1% Inter-AssayCV:?7.8% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate sIgA in samples. An antibody specific for sIgA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anysIgA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for sIgA is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sIgA bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Secretory IgA (IgA) consists of two IgA monomers joined by the J-chain and furthermore a secretory component. It is secreted in plasma cells based in the lamina propia of mucosal membranes. Synthesis of IgA is independent from the synthesis of serum IgA. This means lack of serum IgA does not necessarily mean a lack of IgA. IgA is found in secretions in a specific form called secretory IgA', Secretory IgA is the major immunoglobµLin in saliva, tears, colostrum, nasal mucous, mother´s milk, tracheobronchial and gastrointestinal secretes. It plays a major role in preventing adherence of microorganisms to mucosal sites, in activation of the alternative complement pathway and in activating inflammatory reactions. Newborns are provided with IgA by mother´s milk and are passively immunized against gastrointestinal infections. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).