ELISA Bovine Trimethyllysine dioxygenase, mitochondrial (TMLHE)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:Q0VC74 Abbreviation:TmLHE Alternative Names:BBOX2; FLJ10727; TmLH; XAP130; butyrobetaine (gamma); 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 2|epsilon-trimethyllysine 2-oxoglutarate|epsilon-trimethyllysine hydroxylase Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.5% Inter-AssayCV:?7.8% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TmLHE in samples. An antibody specific for TmLHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTmLHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TmLHE is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TmLHE bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Epsilon-N-trimethyllysine hydroxylase (EC 1.14.11.8) catalyzes the conversion of epsilon-N-trimethyllysine to beta-hydroxy-N-epsilon-trimethyllysine in the first step of L-carnitine biosynthesis. The deduced 421-amino acid TmLHE and 405-amino acid rat TmLhe proteins have calcµLated molecµLar masses of 49.5 kD and 47.5 kD, respectively, and share 92% amino acid identity. SubcellµLar fractionation localized rat TmLhe to mitochondria. Rat TmLhe was syntheQuantityd as a 47.5-kD precursor that is processed to an approximately 43-kD protein, most likely throµgh cleavage of a mitochondrial targeting signal. Gel filtration analysis showed rat TmLhe as an 87-kD band, sµggesting that TmLhe forms a homodimer. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).