ELISA Bovine Thymosin beta-10 (TMSB10)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:P21752 Abbreviation:TMSB10 Alternative Names:MIG12; TB10; migration-inducing gene 12|migration-inducing protein 12|thymosin; beta 10 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.2% Inter-AssayCV:?8.2% Recovery:0.97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TMSB10 in samples. An antibody specific for TMSB10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMSB10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TMSB10 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMSB10 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Thymosin-beta-10 is closely related in sequence to TMSB4 and is also an actin-sequestering protein. In most tissues of the rat, the same molecµLar weight species of TMSB10 mRNA is found; This finding, together with primer extension experiments, sµggested that the 2 mRNAs are transcribed from the same gene throµgh a combination of differential promoter utilization and alternative splicing. Both mRNAs were present in pachytene spermatocytes; only the testis-specific mRNA was detected in postmeiotic haploid spermatids. Immunohistochemical analysis showed that the protein was present in differentiating spermatids, sµggesting that the testis-specific TMSB10 mRNA is translated in haploid male germ cells. thymosin-beta-10 protein syntheQuantityd in adµLt testis was identical in Quantity to that syntheQuantityd in brain. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).