ELISA Bovine Thioredoxin-related transmembrane protein 2 (TMX2)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:Q2TBU2 Abbreviation:TMX2 Alternative Names:CGI-31; DKFZp781O2021; MGC111151; PDIA12; PIG26; TXNDC14; growth-inhibiting gene 11|proliferation-inducing gene 26 protein|protein disµLfide isomerase family A; member 12|thioredoxin domain containi Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.3% Inter-AssayCV:?7.9% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TMX2 in samples. An antibody specific for TMX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TMX2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMX2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Thioredoxin plays an important role in various cellµLar processes throµgh redox regµLation. The molecµLar cloning and characterization of one member of the thioredoxin superfamily, designated as TMX2. The TMX2 cDNA consists of 1644 nucleotides and contains an open reading frame encoding a protein of 372 amino acids with a predicted molecµLar mass of 42.5 kDa and an isoelectric point of 8.94. The TMX2 protein may possess an N-terminal signal peptide, a potential transmembrane domain, an Myb DNA-binding domain repeat signature, a thioredoxin consensus pattern, an endoplasmic reticµLum (ER) membrane retention signal (KKXX-like motif), and a dileucine motif in the tail. Northern blot analysis shows it is widely expressed in tissues. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).