ELISA Bovine Troponin T, cardiac muscle (TNNT2)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:P13789 Abbreviation:TNNT2 Alternative Names:CMH2; CMPD2; MGC3889; RCM3; TnTC; cTnT; OTTHUMP00000033864|OTTHUMP00000033867|cardiomyopathy; dilated 1D (autosomal dominant)|cardiomyopathy; hypertrophic 2|troponin T type 2; cardiac|troponin T; ca Application:ELISA Range:15.6-1000 pg/mL Sensitivity:5.2 pg/mL Intra-AssayCV:?4.3% Inter-AssayCV:?8.1% Recovery:0.96 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TNNT2 in samples. An antibody specific for TNNT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TNNT2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNT2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:TNNT2 is located on the thin filament of striated muscles and regµLates muscle contraction in response to alterations in intracellµLar calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).