ELISA AP2B1 anti-

Quantity :50µL Clone Number: Aliases:Adapter related protein complex 2 beta 1 subunit antibody; Adapter-related protein complex 2 beta subunit antibody; Adaptin, beta 2 (beta) antibody; Adaptor protein complex AP-2 subunit beta antibody; Adaptor related protein complex 2, beta 1 subunit antibody; ADTB2 antibody; AP-2 complex subunit beta antibody; AP105B antibody; AP2 BETA antibody; Ap2b1 antibody; AP2B1_ antibody; Beta adaptin antibody; Beta-2-adaptin antibody; Beta-adaptin antibody; Beta2 adaptin antibody; CLAPB1 antibody; Clathrin assembly protein complex 2 beta large chain antibody; Clathrin associated/assembly/adaptor protein, large, beta 1 antibody; DKFZp781K0743 antibody; Plasma membrane adaptor HA2/AP2 adaptin beta subunit antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P63010 Immunogen:Recombinant AP-2 complex subunit beta protein (738-937AA) Raised in:Rabbit Reactivity:, Mouse Tested Applications:ELISA, WB, IHC; Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200 Background:Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecµLes. AP-2 may also play a role in maintaining normal post-endocytic trafficking throµgh the ARF6-regµLated, non-clathrin pathway. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly. Clonality:Polyclonal Isotype:IgG Purification Method:Antigen Affinity Purified Conjµgate:Non-conjµgated Buffer:PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:AP2B1 Research Areas:Signal transduction