ELISA ADAM23 Antibody, FITC

Overview of ELISA ADAM23

The Enzyme-Linked Immunosorbent Assay (ELISA) for ADAM23 is a sensitive immunoassay designed to quantitatively measure the concentration of ADAM23 protein in biological samples such as serum, plasma, or cell lysates. ADAM23 (A Disintegrin And Metalloproteinase domain-containing protein 23) is a member of the ADAM family involved in cell adhesion and signal transduction processes.

This ELISA utilizes a sandwich format, where specific monoclonal or polyclonal antibodies capture ADAM23 molecules from the sample onto a solid microplate. A detection antibody conjugated to an enzyme, commonly horseradish peroxidase (HRP), binds to a different epitope of ADAM23, forming a complex. Upon addition of a chromogenic substrate (e.g., TMB), the enzyme catalyzes a colorimetric reaction proportional to the amount of ADAM23 present.

The assay typically features:

• High specificity through antibody pairs targeting non-overlapping epitopes on ADAM23.
• Detection range suited for physiological and pathological concentrations, often from low pg/mL to ng/mL levels.
• Sample types: serum, plasma, cell culture supernatants, or tissue homogenates.
• Assay time: approximately 2-4 hours, including incubation and wash steps.
• Quantitative output: optical density measured at 450 nm correlates with ADAM23 concentration via a standard curve.

Applications of ELISA ADAM23 include research on neurological development, tumor biology, and autoimmune diseases, where ADAM23 expression or shedding may be altered. The assay is critical for elucidating the role of ADAM23 in cell adhesion, neuronal signaling, and its potential as a biomarker in disease states.