ELISA CARM1 anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:CARM1, also named as PRMT4, belongs to the protein arginine N-methyltransferase family. It is a dual functional coregµLator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein. CARM1 functions as a coactivator for many nuclear receptors, such as oestrogen receptor, androgen receptor, thyroid receptor and farnesoid X-receptor. It also coactivates other transcription factors such as myocyte enhancer factor 2C (MEF2C), ?-catenin, p53, nuclear factor (NF)-kB and the cAMP-responsive element-binding factor. The enzymatic activity and coactivator function of CARM1 has been found to be inactivated throµgh phosphorylation at a conserved serine residue at mitosis stage. This antibody was generated against a synthetic peptide corresponding to a fragment of CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTMsites in cells themselves. Immunogen:coactivator-associated arginine methyltransferase 1 Synonyms:CARM1, PRMT4 Observed MW:63-66 kDa, 55 kDa Uniprot ID:Q86X55 Reactivity:, Mouse, Rat, Monkey Tested Application:ELISA, IHC, WB, IF Recommended dilution:WB: 1:1000-1:5000; IHC: 1:50-1:500; IF: 1:20-1:200 Gene ID:10498 Research Area:Epigenetics, Metabolism