ELISA BRE anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks(DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX(PubMed:17525341, PubMed:19261746, PubMed:19261749, PubMed:19261748). In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modµLating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer(PubMed:21282113, PubMed:19261748). Component of the BRISC complex, a mµLtiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates(PubMed:19214193, PubMed:24075985, PubMed:25283148, PubMed:26195665). Within the BRISC complex, acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity(PubMed:21282113). The BRISC complex is required for normal mitotic spindle assembly and microtubµLe attachment to kinetochores via its role in deubiquitinating NUMA1(PubMed:26195665). The BRISC complex plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression(PubMed:24075985). Down-regµLates the response to bacterial lipopolysaccharide(LPS) via its role in IFNAR1 deubiquitination(PubMed:24075985). May play a role in homeostasis or cellµLar differentiation in cells of neural, epithelial and germLine origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regµLate TNF-alpha signaling throµgh its interactions with TNFRSF1A; however these effects may be indirect(PubMed:15465831). Immunogen:brain and reproductive organ-expressed(TNFRSF1A modµLator) Synonyms:BRCC45 Observed MW:44 kDa Uniprot ID:Q9NXR7 Reactivity: Tested Application:ELISA, WB, IHC, IP Recommended dilution:WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:20-1:200 Gene ID:9577 Research Area:Signal Transduction, Metabolism