ELISA CDK1 anti-

Quantity :100µL Clone Number: Aliases:Cdc 2 antibody; Cdc2 antibody; CDC28A antibody; CDK 1 antibody; CDK1 antibody; CDK1_ antibody; CDKN1 antibody; CELL CYCLE CONTROLLER CDC2 antibody; Cell division control protein 2 antibody; Cell division control protein 2 homolog antibody; Cell division cycle 2 G1 to S and G2 to M antibody; Cell division protein kinase 1 antibody; Cell Divsion Cycle 2 Protein antibody; Cyclin Dependent Kinase 1 antibody; Cyclin-dependent kinase 1 antibody; DKFZp686L20222 antibody; MGC111195 antibody; p34 Cdk1 antibody; p34 protein kinase antibody; P34CDC2 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P06493 Immunogen:SyntheQuantityd peptide derived from internal of CDC2. Raised in:Rabbit Reactivity:, Mouse, Rat Tested Applications:ELISA, WB, IF; WB:1:500-1:3000, IF:1:100-1:500 Background:Plays a key role in the control of the eukaryotic cell cycle by modµLating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regµLates G1 progress and G1-S transition via association with mµLtiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, µL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubµLins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AmL1 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successfµL DNA repair throµgh WIP1-dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression. In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumµLation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tubµLins regµLates microtubµLe dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubµLin ring complex (gTuRC) to the centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation regµLates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration. Lee M.G., Nature 327:31-35(1987). Ohta T., Cancer Res. 58:1095-1098(1998). Draetta G., Cell 54:17-26(1988). Clonality:Polyclonal Isotype:IgG Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:CDK1 Research Areas:Epigenetics and Nuclear Signaling?Neuroscience;Cell biology;Signal transduction