ELISA APAF1 anti-
Quantity :100µL
Clone Number:
Aliases:APAF1-interacting protein; APIP; APIP2; CGI-29 protein; MMRP19
Product Type:Polyclonal Antibody
Immunogen Species:Homo sapiens ()
UniProt ID:O14727-6
Immunogen:SyntheQuantityd peptide derived from APAF-1-ALT.
Raised in:Rabbit
Reactivity:
Tested Applications:ELISA, WB, IHC, IF; WB:1:500-1:3000, IHC:1:50-1:200, IF:1:100-1:200
Background:This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimµLates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing resµLts in several transcript variants encoding different isoforms.
Keigo Machida, J. Biol. Chem., Apr 2001; 276: 12140 - 12146.
Liam O?nnor, Cancer Res., Mar 2000; 60: 1217 - 1220.
Zeping Wang, Nucleic Acids Res., Apr 2005; 33: 2248 - 2258
Govindaraj Anumanthan, Cancer Res., Nov 2006; 66: 10824 - 10832.
Clonality:Polyclonal
Isotype:IgG
Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjµgate:Non-conjµgated
Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form:liquid
Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names:APAF1
Research Areas:Cancer;Cell biology;Metabolism