ELISA AKT1|AKT2|AKT3 (Ab-315|316|312) anti-

Quantity :100µL Clone Number: Aliases:RAC-PK-alpha; Protein kinase B; Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P31749/P31751/Q9Y243 Immunogen:Peptide sequence around aa.313~317/314~318/310~314 (P-E-Y-L-A) derived from AKT1/AKT2/AKT3. Raised in:Rabbit Reactivity:, Mouse, Rat Tested Applications:ELISA, IHC; IHC:1:50-1:100 Background:General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insµLin and insµLin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insµLin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insµLin-stimµLated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insµLin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. /IGF-1 leads to the activation of AKT3, which may play a role in regµLating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site coµLd still be stimµLated but to a lesser extent. Nelms K, et al. (1999) Annu Rev Immunol. 17:701-738. Malabarba M G, et al. (1996) Biochem. J. 319:865-872. Hou J, et al. (1994) Science. 265:1701-1706. Quelle F W, et al. (1995) Mol Cell Biol. 15: 3336-3343. Takeda K, et al. (1996) Nature. 380: 627-630. Clonality:Polyclonal Isotype:IgG Purification Method:Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjµgates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. Conjµgate:Non-conjµgated Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:AKT1/AKT2/AKT3 Research Areas:Epigenetics and Nuclear Signaling?Neuroscience?Cancer;Metabolism;Signal transduction