ELISA Phospho-Rpb1 CTD (Tyr145) anti-

Quantity:100µL Host:Rabbit Clonality:Polyclonal Isotype:IgG GeneName:POLR2A GeneID:5430 Uniprot:P24928 Specificity:Phospho-Rpb1 CTD (Tyr145) Antibody detects endogenous levels of Rpb1 CTD only when phosphorylated at Tyr145. Immunogen:A syntheQuantityd peptide derived from Rpb1 CTD around the phosphorylation site of Tyr145. Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity:,Mouse,Rat,Monkey Prediction:Pig,Bovine,Horse,Sheep,Dog,Xenopus Application:WB,IHC,ELISA(peptide) Dilution:WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000 Conjµgate:Unconjµgated RRID:AB_2843713 An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide:AF7273-BP Des# Blocking Peptide:Phospho-Rpb1 CTD (Tyr145) Blocking peptide Blocking Peptide Price:$549 CAD