ELISA alpha-SMA anti-

Quantity:100µL Host:Rabbit Clonality:Polyclonal Isotype:IgG GeneName:ACTA2 GeneID:59 Uniprot:P62736 Specificity:alpha-SMA Antibody detects endogenous levels of total alpha-SMA. Immunogen:A syntheQuantityd peptide derived from alpha-SMA, corresponding to a region within N-terminal amino acids. Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 15 months from date of receipt. Reactivity:,Mouse,Rat,Bovine Prediction:Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken Application:WB,IHC,IF/ICC,IP,ELISA(peptide) Dilution:WB: 1:500-1:1000, IHC: 1:50-1:500, IF/ICC: 1:200, IP 1:100, ELISA(peptide) 1:20000-1:40000 Conjµgate:Unconjµgated RRID:AB_2835329 An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide:AF1032-BP Des# Blocking Peptide:alpha-SMA Blocking peptide Blocking Peptide Price:$549 CAD