ELISA Ovalbumin (OVA)

Reactivity:Chicken (Gallus) UniProt:N/A Abbreviation:OVA Alternative Names:N/A Application:ELISA Range:123.5-10000 pg/mL Sensitivity:52.4 pg/mL Intra-AssayCV:?5.2% Inter-AssayCV:?8.6% Recovery:0.96 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate OVA in samples. An antibody specific for OVA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyOVA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for OVA is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OVA bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Ovalbumin is the main protein found in egg white, making up 60-65% of the total protein. While Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, it is a noninhibitory serpin While most serpins control such processes as fibrinolysis and coagµLation by inhibiting serine proteases, the function of ovalbumin is unknown, althoµgh it is presumed to be a storage protein.The ovalbumin protein of chickens is made up of 385 amino acids, and its relative molecµLar mass is 45 kDa. It is a glycoprotein with 4 sites of glycosylation.It is secreted from the cell. Unlike the signal sequences of most secreted proteins, Ovalbumin's signal sequence is not cleaved. Additionally it is not located directly on the N-terminus, but rather internally, starting at residue 21 and ending at residue 47. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).