ELISA Hydroxyacylglutathione hydrolase, mitochondrial (HAGH)

Reactivity:Chicken (Gallus) UniProt:Q5ZI23 Abbreviation:HAGH Alternative Names:GLO2; GLX2; GLXII; HAGH1; glyoxalase II|hydroxyacylglutathione hydroxylase Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.129 ng/mL Intra-AssayCV:?4.6% Inter-AssayCV:?7.4% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate HAGH in samples. An antibody specific for HAGH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHAGH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for HAGH is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAGH bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Glyoxalase II, otherwise known as hydroxyacyl-glutathione hydrolase, converts the intermediate substrate S-lactoyl-glutathione to reduced glutathione and D-lactate. By study of somatic cell hybrids, Honey and Shows (1981) concluded that the gene for glyoxalase II is on chromosome 16. MµLley and Callen (1986) confirmed the assignment of HAGH to chromosome 16 by studies of a -mouse hybrid panel. They found that both HAGH and phosphoglycolate phosphatase (PGP) were present only in those cell lines containing 16p13. Board (1980) described rare polymorphism, observed only in a Micronesian popµLation in which a new variant allele had a frequency of 0.016. In the heterozygotes, the electrophoretic pattern was a double band, sµggesting that the structure of glyoxalase II is monomeric. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).