ELISA Prolyl 3-hydroxylase 2 (LEPREL1)

Reactivity:Chicken (Gallus) UniProt:Q6JHU7 Abbreviation:LEPREL1 Alternative Names:FLJ10718; mLAT4; P3H2; myxoid liposarcoma associated protein 4|prolyl 3-hydroxylase 2|prolyl 3-hydroxylase 3 Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.126 ng/mL Intra-AssayCV:?3.9% Inter-AssayCV:?7.2% Recovery:0.92 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LEPREL1 in samples. An antibody specific for LEPREL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLEPREL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LEPREL1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LEPREL1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:LEPREL1 belongs to a family of collagen prolyl hydroxylases required for proper collagen biosynthesis, folding, and assembly. The deduced 708-amino acid protein has an N-terminal signal peptide, 4 tetratricopeptide repeats, 4 CxxxC motifs, a central leucine zipper, a prolyl 4-hydroxylase-alpha domain, which contains an ATP/GTP-binding site, and a C-terminal endoplasmic reticµLum (ER) retention motif (KDEL). LEPREL1 also has 3 potential N-glycosylation sites and putative glycosaminoglycan attachment sites. It shares 53% and 45% amino acid identity with LEPRE1 and LEPRE2, respectively. Northern blot analysis detected weak expression of a 3.4-kb transcript in most tissues examined, with higher levels in placenta and kidney. A 6.5-kb transcript was expressed abundantly in skeletal muscle and more weakly in heart. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).