ELISA Bovine Cytochrome b (MT-CYB)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:P00157 Abbreviation:MT-CYB Alternative Names:MTCYB; Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.053 ng/mL Intra-AssayCV:?4.2% Inter-AssayCV:?7.5% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate MT-CYB in samples. An antibody specific for MT-CYB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT-CYB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for MT-CYB is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT-CYB bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Mutations in the mitochondrial DNA cytochrome b gene (MTCYB) have been commonly associated with isolated mitochondrial myopathy and exercise intolerance, rarely with mµLtisystem disorders, and only once with a parkinsonism/mitochondrial encephalomyopathy, lactic acidosis, and strokelike episodes (MELAS) overlap syndrome. Defects in MT-CYB are a rare cause of mitochondrial dysfunction underlying different myopathies. Defects in MTCYB are also found in cases of exercise intolerance accompanied by deafness, mental retardation, retinitis pigmentosa, cataract, growth retardation, epilepsy (mµLtisystem disorder). Defects in MT-CYB are the cause of cardiomyopathy infantile histiocytoid (CMIH). Defects in MT-CYB contribute to Leber hereditary optic neuropathy (LHON). Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).