ELISA Cat Acetylcholine receptor subunit alpha (CHRNA1)

Reactivity:Cat (Felis catus,Feline) UniProt:P54250 Abbreviation:CHRNA1 Alternative Names:ACHRA; ACHRD; CHRNA; CMS2A; FCCMS; SCCMS; cholinergic receptor; nicotinic; alpha polypeptide 1 (muscle)|nicotinic acetylcholine receptor alpha subunit|nicotinic cholinergic receptor alpha 1 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.078 ng/mL Intra-AssayCV:?4.9% Inter-AssayCV:?8.5% Recovery:0.79 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate CHRNA1 in samples. An antibody specific for CHRNA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHRNA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for CHRNA1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRNA1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview: Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).