ELISA Bovine Mitotic spindle assembly checkpoint protein MAD2B (MAD2L2)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:Q2KIP7 Abbreviation:MAD2L2 Alternative Names:RP3-330O12.4; MAD2B; REV7; MAD2 (mitotic arrest deficient; yeast; homolog)-like 2|MAD2 homolog|OTTHUMP00000002273|mitotic arrest deficient homolog-like 2 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?6.6% Inter-AssayCV:?9.8% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate MAD2L2 in samples. An antibody specific for MAD2L2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAD2L2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for MAD2L2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAD2L2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1. The predicted 211-amino acid MAD2B protein shares 24% and 26% sequence identity with yeast Mad2 and MAD2L1, respectively, in the conserved regions. RT-PCR analysis revealed that both MAD2 homologs were expressed at similar high levels in a panel of cell lines. Further binding analyses determined that the interaction of MAD2L2 with ADAM9 is mediated throµgh a proline-rich SH3-ligand domain of ADAM9. Northern blot analysis detected 1.35-kb MAD2L5 transcripts in all tissues tested, with highest expression in testis. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).