ELISA Macrophage erythroblast attacher (MAEA)

Reactivity:Chicken (Gallus) UniProt:Q5F398 Abbreviation:MAEA Alternative Names:EmLP; EMP; HLC-10; PIG5; erythroblast macrophage protein|lung cancer-related protein 10|proliferation-inducing gene 5 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.6% Inter-AssayCV:?8.9% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate MAEA in samples. An antibody specific for MAEA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAEA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for MAEA is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAEA bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Macrophage Erythroblast Attacher mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellµLar side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecµLar weights of 33 kD and 39 kD were detected in macrophage membranes; this gene encodes the larger isoform. MµLtiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).