ELISA Bovine Methionine adenosyltransferase 2 subunit beta (MAT2B)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:Q29RI9 Abbreviation:MAT2B Alternative Names:MAT-II; MATIIbeta; MGC12237; Nbla02999; SDR23E1; TGR; beta regµLatory subunit of methionine adenosyltransferase|dTDP-4-keto-6-deoxy-D-glucose 4-reductase|putative protein product of Nbla02999|short Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?7.6% Inter-AssayCV:?10.5% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate MAT2B in samples. An antibody specific for MAT2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAT2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for MAT2B is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAT2B bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Methionine adenosyltransferase (MAT; EC 2.5.1.6) catalyzes the biosynthesis of S-adenosylmethionine (AdoMet) from methionine and ATP. MAT II is a broadly expressed MAT consisting of catalytic alpha and noncatalytic beta subunits encoded by MAT2A) and MAT2B, respectively.LeGros et al. (2000) designed degenerate PCR primers to tryptic peptide sequence of MAT2B purified from lymphocytes. They amplified a partial cDNA from Jurkat cells and extended the cDNA using 3-prime and 5-prime RACE. A fµLl-length MAT2B cDNA amplified from normal peripheral blood mononuclear cell RNA encodes a 334-amino acid protein with a molecµLar mass of 38 kD. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).