ELISA Bovine CAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B)

Reactivity:Bovine (Bos taurus; Cattle) UniProt:P31322 Abbreviation:PRKAR2B Alternative Names:PRKAR2; RII-BETA; H_RG363E19.2|WµgSC:H_RG363E19.2|cAMP-dependent protein kinase type II-beta regµLatory chain|cAMP-dependent protein kinase; regµLatory subunit beta 2 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?6.1% Inter-AssayCV:?8.4% Recovery:1.1 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PRKAR2B in samples. An antibody specific for PRKAR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PRKAR2B is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR2B bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The inactive holoenzyme of AMPK is a tetramer composed of two regµLatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regµLatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regµLatory subunits and three catalytic subunits of AMPK have been identified in s. PRKAR2b is one of the regµLatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice sµggest that this subunit may play an important role in regµLating energy balance and adiposity. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).